Microarray results: how accurate are they?
作者： Ravi Kothapalli , Sean J Yoder , Shrikant Mane , Thomas P Loughran
关键词: Computational biology 、 Gene expression profiling 、 Genetics 、 Biology 、 Oligonucleotide 、 Microarray analysis techniques 、 DNA microarray 、 Microarray databases 、 Microarray 、 Complementary DNA 、 Gene chip analysis
摘要: DNA microarray technology is a powerful technique that was recently developed in order to analyze thousands of genes short time. Presently, microarrays, or chips, the cDNA type and oligonucleotide are available from several sources. The number publications this area increasing exponentially. In study, data obtained two different commercially systems were critically evaluated. Our analysis revealed inconsistencies microarrays. Problems encountered included inconsistent sequence fidelity spotted variability differential expression, low specificity probes, discrepancy fold-change calculation lack probe for isoforms gene. view these pitfalls, need be interpreted cautiously.
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