An alternative method for the isolation of mesenchymal stromal cells derived from lipoaspirate samples
作者： Leandra S Baptista , Ronaldo JF C do Amaral , Rosana BV Carias , Marcelo Aniceto , Cesar Claudio-da-Silva
DOI: 10.3109/14653240902981144
关键词: Mesenchymal stem cell 、 In vitro 、 Cell biology 、 Immunology 、 Cell 、 Chemistry 、 Population 、 Cryopreservation 、 Tissue engineering 、 Stromal cell 、 Adipose tissue
摘要: Background aimsSince initial methods were developed for isolating cells from adipose tissue, little has been done to improve mesenchymal stromal cell (MSC) yield. The aim of the present study was isolate a population MSC lipoaspirate samples without tissue digestion and assess possibility cryopreserving freshly isolated cells.MethodsA 13 patients’ by mechanical dissociation. Mechanically processed lipoaspi-rate (MPLA) characterized after in vitro expansion morphologic analysis, expression surface markers differentiation assays.ResultsMechanical dissociation yielded large quantity adherent both standard vibro-assisted liposuction. Preservation at 4°C 1 or 2 days until procedure did not change MPLA content. It possible store cryopreservation loss population...
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