Protective Effect of Saccharomyces boulardii on Deoxynivalenol-Induced Injury of Porcine Macrophage via Attenuating p38 MAPK Signal Pathway
作者： Chao Chang , Kun Wang , Sheng-Nan Zhou , Xue-Dong Wang , Jin-E Wu
DOI: 10.1007/S12010-016-2335-X
关键词: Saccharomyces boulardii 、 Protein kinase A 、 Gene expression 、 Alveolar macrophage 、 Molecular biology 、 p38 mitogen-activated protein kinases 、 Proinflammatory cytokine 、 Apoptosis 、 Biology 、 Western blot
摘要: The aims of our study were to evaluate the effects Saccharomyces boulardii (S. boulardii) on deoxynivalenol (DON)-induced injury in porcine alveolar macrophage cells (PAMCs) and explore underlying mechanisms. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis, ELISA, qRT-PCR, western blot performed assess whether S. could prevent DON-induced by p38 mitogen-activated protein kinase (p38 MAPK) signal pathway. results showed that pretreatment with 8 μM DON decrease viability PAMC significantly increase apoptosis rate PAMC, whereas rescue apoptotic induced DON. Further experiments revealed effectively reversed cytotoxicity via downregulating expression TNF-α, IL-6, IL-lβ. In addition, alleviated phosphorylation mRNA further increased regulation genes Bcl-xl Bcl-2 inhibited activation Bax. Our suggest suppress MAPK pathway reduce downstream inflammatory cytokines, as well promote anti-apoptotic inhibit PAMC.
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