Characterization of sugars from model and enzyme-mediated pulp hydrolyzates using high-performance liquid chromatography coupled to evaporative light scattering detection.

作者: Paul B. Filson , Benjamin E. Dawson-Andoh

DOI: 10.1016/J.BIORTECH.2008.12.067

关键词: HydrolysisPulp (paper)Chromatography detectorChromatographyHigh-performance liquid chromatographyCellobioseCellulaseChemistryCelluloseSugar

摘要: High-performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector was used quantitatively determine glucose and cellobiose in hydrolyzates from the production of cellulose nanofillers modified lignocellulosic materials. Prevail Carbohydrate ES 5 micron column proved more suitable for achieving chromatographic separation model pulp hydrolyzate into its constituent sugars than YMC-Pack Polyamine column. Linear calibration curves various mixtures were developed. Glucose clearly detectable obtained enzyme-mediated hydrolysis recycled pulp, pine hardwood dissolving pulps. Finally, amount generally higher cellobiose.

参考文章(19)
Ingvild Kvien, Junji Sugiyama, Martin Votrubec, Kristiina Oksman, Characterization of starch based nanocomposites Journal of Materials Science. ,vol. 42, pp. 8163- 8171 ,(2007) , 10.1007/S10853-007-1699-2
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
S.G. Sunderwirth, Gary G. Olson, Gestur Johnson, PAPER CHROMATOGRAPHY-ANTHRONE DETERMINATION OF SUGARS. Journal of Chromatography A. ,vol. 16, pp. 176- 180 ,(1964) , 10.1016/S0021-9673(01)82452-3
Qiuju Wu, Marielle Henriksson, Xiaohui Liu, Lars A. Berglund, A High Strength Nanocomposite Based on Microcrystalline Cellulose and Polyurethane Biomacromolecules. ,vol. 8, pp. 3687- 3692 ,(2007) , 10.1021/BM701061T
M.Mı́guez Bernárdez, J De la Montaña Miguélez, J.Garcı́a Queijeiro, HPLC determination of sugars in varieties of chestnut fruits from Galicia (Spain) Journal of Food Composition and Analysis. ,vol. 17, pp. 63- 67 ,(2004) , 10.1016/S0889-1575(03)00093-0
Magdolna Morvai, Ibolya Molnár-Perl, Dezsó Knausz, Simultaneous gas-liquid chromatographic determination of sugars and organic acids as trimethylsilyl derivatives in vegetables and strawberries Journal of Chromatography A. ,vol. 552, pp. 337- 344 ,(1991) , 10.1016/S0021-9673(01)95950-3
Tao Pang, Changmin Bai, Yanjuan Xu, Guowang Xu, Zhongyi Yuan, Yong Su, Liming Peng, Determination of Sugars in Tobacco Leaf by HPLC with Evaporative Light Scattering Detection Journal of Liquid Chromatography & Related Technologies. ,vol. 29, pp. 1281- 1289 ,(2006) , 10.1080/10826070600598993
Neng Wang, Enyong Ding, Rongshi Cheng, Preparation and liquid crystalline properties of spherical cellulose nanocrystals. Langmuir. ,vol. 24, pp. 5- 8 ,(2008) , 10.1021/LA702923W