Insulin-stimulated glycogen formation in rat diaphragm. Levels of tissue intermediates in short-time experiments.

摘要: Abstract Studies carried out with rat hemidiaphragms indicated that, under the experimental conditions outlined, over 90% of increased uptake glucose in presence insulin, on a balance basis, could be accounted for as tissue glycogen. Hexose 6-phosphate content was diaphragms incubated agreement an conversion extracellular to intracellular hexose 6-phosphate. Glucose 1-phosphate contents were unchanged, suggesting rapid removal 1-phosphate. Glycogen formation insulin took place at ratios inorganic phosphate 305. ADP, ATP, phosphate, and creatine not changed, finding compatible association glycogen phosphorylative reactions.