Cell-Cycle Analysis in 20 Minutes
作者： H. A. Crissman , R. A. Tobey
DOI: 10.1126/SCIENCE.184.4143.1297
关键词: DNA 、 Aqueous ethanol 、 Analytical chemistry 、 Cellular dna 、 Flow system 、 Biophysics 、 Cell cycle analysis 、 Quantitative fluorescence 、 Biology 、 Kinetics
摘要: Mithramycin added to mammalian cells fixed in aqueous ethanol is bound DNA and fluoresces direct proportion the cellular content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides rapid method for cell-cycle analysis first time permits continuous monitoring kinetics during ongoing experiments.
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harvard.edu参考文章(6)
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