Flow Cytometry and Cell Sorting

摘要: Characterization of cell populations has always been a primary interest biologists. Traditionally, some kind microscopy—combined with biochemical or immunological techniques—was used to discriminate different types otherwise characterize new population. Microscopy gives very accurate morphological information about the cells, and it may also be for quantitative measurement cellular properties. However, these measurements are time-consuming, so accuracy determined parameter is usually limited by low number cells observed consequently high statistical errors. Rare variants remain undetected in such analyses. The drawbacks microscopy can overcome automated sampling populations. first big step this direction was made Caspersson et al., who solved problem measuring DNA content on slides automatically. High-resolution image analysis developed from his approach. A second approach cytology suspension flowing past detector, known as flow cytometry. Flow cytometry now widely applied tool biology immunology well clinical laboratories, though its potential characterization far fully realized. This chapter deals cytometric In we summarize advances instrumentation methodology discuss recent applications method biophysics.