Technique for cellular fluorescence distribution analysis
作者： R. D. Robinson , J. E. Reeder , L. L. Wheeless
关键词:
摘要: The usefulness of multidimensional slit-scan flow cytometry in whole cell measurements is dependent on extracting relevant features from the cellular fluorescence distributions (slit-scan contours). In addition, extraction these must be rapid to allow for realtime data processing during acquisition. This paper describes two algorithms that have been used successfully count numbers local maxima (peaks) and find nuclear bloundaries a distribution. These routines are efficient, use only simple integer arithmetic, implemented several different microprocessors.
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