System for acquisition and real-time processing of multidimensional slit-scan flow cytometric data

摘要: The high-speed sampling requirements of multidimensional slit-scan signals (cell contours) have typically required custom hardware. This specialized hardware has often lacked the flexibility to adapt varying instrument setups and experimental requirements. A software system capable multiple cell contours at rates up 40 MHz with 10-bit resolution is described. It utilizes commercially available CAMAC transient recorders, a Digital Equipment Corp. PDP-11/83 computer, for signal conditioning trigger generation. modular design allows various options minimal additional coding. Real-time digital processing checks each contour peaks; extracts morphological features such as width, height, area; accumulates gated histograms these data; optionally saves derived data, selected contours, or both into list mode files on disk.