Cytofluorometric and cytochemical comparisons of normal and abnormal human cells from the female genital tract.

摘要: Acridine orange staining of exfoliated cells from epithelial tissues facilitates discrimination between normal and abnormal cells: develop highly elevated nuclear fluorescence. Comparisons acridine (AO) with propidium iodide (P1) or Feulgen have shown that: (a) P1 also provides fluorescence cells; (b) the distributions following AO were usually not significantly different as judged by Kolmogorov-Smirnov test; (c) emission spectra stained are consistent hypothesis that both fluorochromes bind to DNA within cell nuclei; (d) DNAse treatment eliminates peak slit-scan contours; RNAse has no effect on fluorescence; (e) distribution after is usually, but always, absorbance staining, relative being greater than absorbance. The currently most these results content can account for only part provided chromatin altered so increase accessibility intercalating dyes.