Cell Sizing: A Light Scattering Photometer for Rapid Volume Determination

作者: P. F. Mullaney , M. A. Van Dilla , J. R. Coulter , P. N. Dean

DOI: 10.1063/1.1684143

关键词: Multiangle light scatteringLight scatteringRayleigh scatteringScatteringMaterials scienceStatic light scatteringPhotometerParticleAnalytical light scatteringOptics

摘要: Theory predicts that small angle light scattering by spherical particles of 5 to 20 μ diam is nearly proportional volume and insensitive particle refractive index. A flow system photometer with helium‐neon laser source measures the between 0.5 2.0° from individual at 104 105/min. Volume distributions mammalian cells plastic microspheres agree other independent determinations.

参考文章(10)
Seibert Q. Duntley, Light in the Sea* Journal of the Optical Society of America. ,vol. 53, pp. 214- 233 ,(1963) , 10.1364/JOSA.53.000214
L. A. Kamentsky, M. R. Melamed, H. Derman, Spectrophotometer: New Instrument for Ultrarapid Cell Analysis Science. ,vol. 150, pp. 630- 631 ,(1965) , 10.1126/SCIENCE.150.3696.630
M. J. Fulwyler, Electronic Separation of Biological Cells by Volume Science. ,vol. 150, pp. 910- 911 ,(1965) , 10.1126/SCIENCE.150.3698.910
P. J. CROSLAND-TAYLOR, A Device for Counting Small Particles suspended in a Fluid through a Tube Nature. ,vol. 171, pp. 37- 38 ,(1953) , 10.1038/171037B0
Gustav Mie, Beiträge zur Optik trüber Medien, speziell kolloidaler Metallösungen Annalen der Physik. ,vol. 330, pp. 377- 445 ,(1908) , 10.1002/ANDP.19083300302
J. H. Tjio, Theodore T. Puck, GENETICS OF SOMATIC MAMMALIAN CELLS : II. CHROMOSOMAL CONSTITUTION OF CELLS IN TISSUE CULTURE Journal of Experimental Medicine. ,vol. 108, pp. 259- 268 ,(1958) , 10.1084/JEM.108.2.259
M. A. Van Dilla, T. T. Truiullo, P. F. Mullaney, J. R. Coultex, Cell Microfluorometry: A Method for Rapid Fluorescence Measurement Science. ,vol. 163, pp. 1213- 1214 ,(1969) , 10.1126/SCIENCE.163.3872.1213
Hendrik Christoffel van de Hulst, Light Scattering by Small Particles ,(1981)
M. A. Van Dilla, M. J. Fulwyler, I. U. Boone, Volume distribution and separation of normal human leucocytes. Experimental Biology and Medicine. ,vol. 125, pp. 367- 370 ,(1967) , 10.3181/00379727-125-32093