GENETICS OF SOMATIC MAMMALIAN CELLS : II. CHROMOSOMAL CONSTITUTION OF CELLS IN TISSUE CULTURE

摘要: A convenient, reliable method for chromosome delineation of animal cells grown as monolayers on glass has been applied to human, opossum, and Chinese hamster cells. Tissue cultured from 5 different, normal organs 7 different human subjects uniformly displayed the expected number 46 showed no variations in morphology or other than sex differences a small incidence polyploidy. The chromosomes American opossum were uniform those Cells inbred demonstrated appreciable karyotype variability, cause which is under investigation. tissues have remained constant after more months continuous, rapid growth tissue culture involving scores vessel transfers generations equivalent many billions progeny. By use routine recloning, even malignant, aneuploid constitution maintained active 3 years hundreds generations, with stable chromosomal metabolic characteristics. possess only 22 established vitro are especially suitable genetic studies. readily recognizeable Y X male particularly favorable cytological markers. Photomicrographs various employed presented.