Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome.
作者： J. Wolfe , S.M. Darling , R.P. Erickson , I.W. Craig , V.J. Buckle
DOI: 10.1016/0022-2836(85)90234-7
关键词: Sequence analysis 、 X chromosome 、 Y chromosome 、 Molecular biology 、 Southern blot 、 Genetics 、 EcoRI 、 Chromosome 22 、 Cosmid 、 Autosome 、 Biology
摘要: A collection of human Y-derived cosmid clones was screened with a plasmid insert containing member the X chromosome alphoid repeat family, DXZ1. Two positive cosmids were isolated and repeats they contained investigated by Southern blotting, in situ hybridization sequence analysis. On to genomic DNAs, expected cross-hybridization characteristic all sequences seen and, addition, 5500 base EcoRI fragment found be Y-specific repeat. Dosage experiments demonstrated that there are about 100 copies this on Y chromosome. Studies utilizing DNA from human-mouse hybrids only portions hybridizations spreads centromeric localization Cross-hybridization autosomes 13, 14 15 also seen; however, these chromosomes lacked detectable arrangement. Sequence analysis DXZ1 70% homology each other consensus sequence. The not gorilla, orangutan or chimpanzee male DNA.
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