CE-ESI-MS metabolic fingerprinting of Leishmania resistance to antimony treatment.

摘要: Metabolomics has become an invaluable tool to unveil biology of pathogens, with immediate application chemotherapy. It is currently accepted that there not one single technique capable obtaining the whole metabolic fingerprint a biological system either due their different physical-chemical properties or concentrations. In this work, we have explored capability capillary electrophoresis mass spectrometry sheathless interface electrospray ionization (CE-ESI-TOF-MS) separate metabolites in order be used as complementary LC. As proof concept, compared metabolome Leishmania infantum promastigotes BCN 150 (Sb (III) IC(50) = 20.9 μM) and its variation when treated 120 μM Sb(III) potassium tartrate for 12 h, well resistant counterpart obtained by growth parasites under increasing step-wise manner up 180 μM. The number were 264 BCN150 versus nontreated 195 susceptible parasites. After successive data filtering, differences seven identified databases pathways, showed highest significant differences, corresponding mainly amino acids metabolite surrogates. Most them assigned sulfur containing polyamine biosynthetic special relevance considering deterioration thiol-dependent redox metabolism Sb(III). Given low concentrations typical most these metabolites, assay can considered success should new questions.