Ionic Liquid Pretreatment of Rice Straw to Enhance Saccharification and Bioethanol Production

作者: Nafiseh Poornejad , Keikhosro Karimi , Tayebeh Behzad

DOI: 10.11159/JBB.2014.002

关键词: FermentationHydrolysisBiofuelChemistryAgronomyEthanol fuelFood scienceEnzymatic hydrolysisStrawCelluloseCellulase

摘要: In this study, rice straw was pretreated by an ionic liquid, 1-ethyl-3-methylimidazolium acetate ((EMIM)(OAc)), prior to enzymatic hydrolysis and ethanol production. The pretreatment carried out at 120 °C for 5 h under atmospheric pressure. Afterward, saccharification conducted 45 72 using 20 FPU cellulase 30 IU β- glucosidase per gram of cellulose. Glucose yield significantly increased from 25.7% the untreated over 75% treated straw. Moreover, simultaneous fermentation improved 35.6% 79.7% SEM images showed more open accessible structure compared one which assisted penetration hydrolytic enzymes in fermentation. From FTIR analysis, it suggested that crystallinity reduction conversion cellulose I II are main changes as a result pretreatment.

参考文章(32)
Cui-Zhi Xu, Lu Li, Shi-Tao Yu, Cong-Xia Xie, Fu-Sheng Liu, EFFICIENT ENZYMATIC IN SITU SACCHARIFICATION OF CELLULOSE IN AQUEOUS-IONIC LIQUID MEDIA BY MICROWAVE PRETREATMENT Bioresources. ,vol. 6, pp. 4494- 4504 ,(2011) , 10.15376/BIORES.6.4.4494-4504
Ning Sun, Mustafizur Rahman, Ying Qin, Mirela L. Maxim, Héctor Rodríguez, Robin D. Rogers, Complete dissolution and partial delignification of wood in the ionic liquid 1-ethyl-3-methylimidazolium acetate Green Chemistry. ,vol. 11, pp. 646- 655 ,(2009) , 10.1039/B822702K
M.J. Negro, P. Manzanares, J.M. Oliva, I. Ballesteros, M. Ballesteros, Changes in various physical/chemical parameters of Pinus pinaster wood after steam explosion pretreatment Biomass and Bioenergy. ,vol. 25, pp. 301- 308 ,(2003) , 10.1016/S0961-9534(03)00017-5
Hua Zhao, Cecil L. Jones, Gary A. Baker, Shuqian Xia, Olarongbe Olubajo, Vernecia N. Person, Regenerating cellulose from ionic liquids for an accelerated enzymatic hydrolysis. Journal of Biotechnology. ,vol. 139, pp. 47- 54 ,(2009) , 10.1016/J.JBIOTEC.2008.08.009
Eduardo A. Ximenes, Carlos R. Felix, Cirano J. Ulhoa, Production of Cellulases by Aspergillus fumigatus and Characterization of One β-Glucosidase. Current Microbiology. ,vol. 32, pp. 119- 123 ,(1996) , 10.1007/S002849900021
Marzieh Shafiei, Hamid Zilouei, Akram Zamani, Mohammad J. Taherzadeh, Keikhosro Karimi, Enhancement of ethanol production from spruce wood chips by ionic liquid pretreatment Applied Energy. ,vol. 102, pp. 163- 169 ,(2013) , 10.1016/J.APENERGY.2012.05.060
Jin Seop Bak, Ja Kyong Ko, Young Hwan Han, Byung Cheol Lee, In-Geol Choi, Kyoung Heon Kim, Improved enzymatic hydrolysis yield of rice straw using electron beam irradiation pretreatment Bioresource Technology. ,vol. 100, pp. 1285- 1290 ,(2009) , 10.1016/J.BIORTECH.2008.09.010
Qiang Yu, Xinshu Zhuang, Zhenhong Yuan, Qiong Wang, Wei Qi, Wen Wang, Yu Zhang, Jingliang Xu, Huijuan Xu, Two-step liquid hot water pretreatment of Eucalyptus grandis to enhance sugar recovery and enzymatic digestibility of cellulose. Bioresource Technology. ,vol. 101, pp. 4895- 4899 ,(2010) , 10.1016/J.BIORTECH.2009.11.051