In vitro activation of the alternative pathway of complement by settled grain dust

摘要: Settled grain dust was collected from several active elevators in the Superior-Duluth areas of United States. Particle size distribution (47% less than 5 micrometer) and endotoxin contamination (429 ng/gm) were similar to those reported for airborne parent dust. Human complement uas activated vitro a dose-response manner which could be quantified. This hemolytic consumption via alternative pathway as defined by ethylenediaminetertraacetic acid/ethylene-glycol-bis-(beta-amino-ethyl ether) N,N'-tetraacetic acid (EDTA/EGTA) differential serum chelation, factor B conversion, reductions guinea pigs deficient C4. It is proposed that continuous low-dose exposure aerosolized, biologically rafter contribute respiratory insult workers.