Differentiation Induction of Human Keratinocytes by Phosphatidylethanolamine-binding Protein
作者： Takehiko Yamazaki , Hajime Nakano , Makoto Hayakari , Masanori Tanaka , Jun Mayama
关键词: Cellular differentiation 、 Cell growth 、 HaCaT 、 Involucrin 、 Kinase 、 Molecular biology 、 Protein kinase A 、 MAPK/ERK pathway 、 Biology 、 Cell culture 、 Cell biology 、 Biochemistry
摘要: Phosphatidylethanolamine-binding protein (PEBP) has been demonstrated to bind Raf-1 and mitogen-activated kinase kinase, components of the extracellular signal-regulated (ERK) pathway, thereby inhibiting pathway resulting in suppression cell proliferation. In present study, we examined whether PEBP is involved differentiation induction human keratinocytes. expression was immunohistochemically normal skin cancers with different properties. not expressed basal layer epidermis but spinous granular layers skin. The differentiated undifferentiated carcinoma. also cultured epidermal keratinocytes which induced by calcium treatment. Involucrin used as a marker for cells. Northern blotting analysis indicated that both involucrin mRNAs were enhanced 6 h after treatment 2.0 mM CaCl(2). amount increased this To investigate keratinocytes, HaCaT transfected an vector. Fluorescent immunostain revealed cells expressing exhibited enlarged flattened shape, immunoblot analysis. Although ERK altered, phosphorylated levels decreased proliferation partly inhibited expression. These results indicate only inhibits induces
highwire.org参考文章(55)
James G. Rheinwald, Chapter 15 Serial Cultivation of Normal Human Epidermal Keratinocytes Methods in Cell Biology. ,vol. 21, pp. 229- 254 ,(1980) , 10.1016/S0091-679X(08)60769-4
James E. Ferrell, MAP kinases in mitogenesis and development. Current Topics in Developmental Biology. ,vol. 33, pp. 1- 60 ,(1996) , 10.1016/S0070-2153(08)60336-1
Randall D. York, Hong Yao, Tara Dillon, Cindy L. Ellig, Stephani P. Eckert, Edwin W. McCleskey, Philip J. S. Stork, Rap1 mediates sustained MAP kinase activation induced by nerve growth factor Nature. ,vol. 392, pp. 622- 626 ,(1998) , 10.1038/33451
R. L. Eckert, J. F. Crish, N. A. Robinson, The epidermal keratinocyte as a model for the study of gene regulation and cell differentiation Physiological Reviews. ,vol. 77, pp. 397- 424 ,(1997) , 10.1152/PHYSREV.1997.77.2.397
Masao Saitoh, Hideki Nishitoh, Makiko Fujii, Kohsuke Takeda, Kei Tobiume, Yasuhiro Sawada, Masahiro Kawabata, Kohei Miyazono, Hidenori Ichijo, Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1. The EMBO Journal. ,vol. 17, pp. 2596- 2606 ,(1998) , 10.1093/EMBOJ/17.9.2596
Kristina Lorenz, Martin J. Lohse, Ursula Quitterer, Protein kinase C switches the Raf kinase inhibitor from Raf-1 to GRK-2. Nature. ,vol. 426, pp. 574- 579 ,(2003) , 10.1038/NATURE02158
P. Arcari, R. Martinelli, F. Salvatore, The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species Nucleic Acids Research. ,vol. 12, pp. 9179- 9189 ,(1984) , 10.1093/NAR/12.23.9179
Ida Bernier, Pierre Jollés, Purification and characterization of a basic 23 kDa cytosolic protein from bovine brain Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. ,vol. 790, pp. 174- 181 ,(1984) , 10.1016/0167-4838(84)90221-8
Anne Deucher, Tatiana Efimova, Richard L. Eckert, Calcium-dependent Involucrin Expression Is Inversely Regulated by Protein Kinase C (PKC)α and PKCδ Journal of Biological Chemistry. ,vol. 277, pp. 17032- 17040 ,(2002) , 10.1074/JBC.M109076200
Marc Schmidt, Matthias Goebeler, Guido Posern, Stephan M. Feller, Cornelia S. Seitz, Eva-B. Bröcker, Ulf R. Rapp, Stephan Ludwig, Ras-independent activation of the Raf/MEK/ERK pathway upon calcium-induced differentiation of keratinocytes. Journal of Biological Chemistry. ,vol. 275, pp. 41011- 41017 ,(2000) , 10.1074/JBC.M003716200