Behavior and Brain Gene Expression Changes in Mice Exposed to Preimplantation and Prenatal Stress

摘要: Preimplantation culture of mouse embryos has been suggested to result in reduced anxiety-like behavior adulthood. Here, we investigated the effects vitro fertilization (IVF), embryo culture, and different diets on using elevated plus maze (EPM). We hypothesized that exposure suboptimal conditions during preimplantation stage would interact with diet alter behavior. The expression genes related anxiety was then assessed by quantitative real-time polymerase chain reaction various brain regions. When fed a normal gestation moderately high-fat Western (WD) postnatally, naturally conceived (NC) IVF mice showed similar EPM. However, when low-protein prenatally postnatally (LP/HF), NC modest increase behavior, whereas opposite: strongly robust reduction males LP/HF was, intriguingly, associated MAO-A, CRFR2, GABA markers hypothalamus cortex. These findings are discussed relation developmental origin health disease hypothesis 2-hit model, which suggests 2 events, occurring at times development, can act synergistically long-term consequences observed