Protocatechuic Acid, a Novel Active Substance against Avian Influenza Virus H9N2 Infection

摘要: Influenza virus H9N2 subtype has triggered co-infection with other infectious agents, resulting in huge economical losses the poultry industry. Our current study aims to evaluate antiviral activity of protocatechuic acid (PCA) against a virulent strain mouse model. 120 BALB/c mice were divided into one control group, untreated 50 mg/kg amantadine hydrochloride-treated group and three PCA groups treated 12 hours post-inoculation 40, 20 or 10 for 7 days. All infected animals inoculated intranasally 0.2 ml A/Chicken/Hebei/4/2008(H9N2) inoculum. A significant body weight loss was found 40 PCA-treated as compared group. The 14 day survivals 94.4%, 100% 95% 94.4% hydrochloride less than 60% Virus loads amantadine-treated groups. Neutrophil cells BALF significantly decreased while IFN-γ, IL-2, TNF-α IL-6 at days Furthermore, CD4+/CD8+ ratio an increased proportion CD19 observed Mice administered exhibited higher survival rate greater viral clearance associated inhibition inflammatory cytokines activation CD8+ T cell subsets. is promising novel agent bird flu infection