RNA interference with EAG1 enhances interferon gamma injury to glioma cells in vitro.
作者： Ricardo Titze-de-Almeida , Walter Stühmer , Luis Pardo , Elaine Del Bel , Ludmylla Costa Cunha
DOI:
关键词: Glioma 、 Chemistry 、 Cancer research 、 RNA interference 、 Gene silencing 、 Real-time polymerase chain reaction 、 Interferon gamma 、 Viability assay 、 Small interfering RNA 、 Messenger RNA
摘要: AIM The aim of this study was to silence Ether a go-go 1 (EAG1) in glioma cells by RNAi order further analyze whether silencing channel would improve injury caused interferon gamma (IFN-γ). MATERIALS AND METHODS EAG1 the siRNAs EAG1hum_287 and EAG1hum_1727 (sequence targets 5'-GGCCTATTGTGTACAGCAATT-3' 5'-GGGACTTCCTGAAGCTCTATT-3', respectively) determined reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR). Cell viability measured 3-(4,5)-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. U-138MG were injured IFN-γ (25 ng/ml, 24 h) with or without for non-viral vector (pKV10.1-3, 0.2 μg). RESULTS 0.46- 0.52-fold decrease mRNA content, respectively. pKv10.1-3 strengthened reduction cell (11.4% versus 40.4%, p<0.05). CONCLUSION present reinforces notion that has role biology, suggesting is relevant player preserving during injury.
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