Delivery of miRNA-Targeted Oligonucleotides in the Rat Striatum by Magnetofection with Neuromag®.

作者: Simoneide Titze de Almeida , Camila Horst , Cristina Soto-Sánchez , Eduardo Fernandez , Ricardo Titze de Almeida

DOI: 10.3390/MOLECULES23071825

关键词: RNA interferenceSense (molecular biology)NeuroprotectionGene expressionmicroRNAOligonucleotideFluorescein isothiocyanateChemistryCell biologyStriatum

摘要: MicroRNAs (miRNAs) regulate gene expression at posttranscriptional level by triggering RNA interference. In such a sense, aberrant expressions of miRNAs play critical roles in the pathogenesis many disorders, including Parkinson’s disease (PD). Controlling specific brain is thus promising therapeutic strategy for neuroprotection. A fundamental need miRNA regulation (either replacing or inhibition) carrier capable delivering oligonucleotides into cells. This study aimed to examine polymeric magnetic particle, Neuromag®, delivery synthetic inhibitors rat central nervous system. We injected inhibitor complexed with Neuromag® lateral ventricles next striatum, stereotaxic surgery. Neuromag efficiently delivered striatum and septum areas, as shown microscopy imaging fluorescein isothiocyanate (FITC)-labeled oligos astrocytes neurons. Transfected showed efficacy concerning inhibition. Neuromag®-structured miR-134 antimiR (0.36 nmol) caused significant 0.35 fold decrease striatal miR-134, revealed real-time quantitative polymerase chain reaction (RT-qPCR). conclusion, particle functional regions surrounding ventricles, particularly striatum. system holds potential miRNA-based disease-modifying drug merits further pre-clinical studies using animal models PD.

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