Detection and Identification of Mycobacterium Species Using Gene Amplification Techniques

摘要: Gene amplification techniques or polymerase chain reactions (PCR) are laboratory procedures that use oligonucleotide primers to direct the of a particular, hopefully diagnostically informative, target sequence detectable level (reviewed in Mullis and Faloona 1987). Basically, these techniques, one begins by denaturing genomic DNA produce single strands DNA. Next, bound complementary sequences on The primer-target complexes then elongated two copies target. Importantly, can only elongate replicate which primer is bound, so specificity process determined primarily used. Finally, sample subjected repeated cycles denaturation, binding, elongation accomplish sequence. Since each cycle results twofold increase sequence, this procedure capable amplifying specific several-million-fold about 25 cycles. Theoretically, such an should allow detection sample.