A rapid method for the detection of potentially viable Mycobacterium leprae in human biopsies: a novel application of PCR.

作者: Sally A. Woods , Stewart T. Cole

DOI: 10.1111/J.1574-6968.1989.TB03678.X

关键词: BiopsyMicrobiologyLeprosyBiologyPolymerase chain reactionMycobacterium lepraeArmadilloIn vitro

摘要: A simple procedure based on the polymerase chain reaction has been developed to detect Mycobacterium leprae, rapidly and unambiguously, in biological samples. Its application small numbers of M. leprae cells (approximately 10(2] isolated from armadillo liver, mouse footpads or human biopsies is discussed.

参考文章(9)
J E Clark-Curtiss, W R Jacobs, M A Docherty, L R Ritchie, R Curtiss, Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae. Journal of Bacteriology. ,vol. 161, pp. 1093- 1102 ,(1985) , 10.1128/JB.161.3.1093-1102.1985
Charles C. Shepard, THE EXPERIMENTAL DISEASE THAT FOLLOWS THE INJECTION OF HUMAN LEPROSY BACILLI INTO FOOT-PADS OF MICE Journal of Experimental Medicine. ,vol. 112, pp. 445- 454 ,(1960) , 10.1084/JEM.112.3.445
Alec J. Jeffreys, Victoria Wilson, Rita Neumann, John Keyte, Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells Nucleic Acids Research. ,vol. 16, pp. 10953- 10971 ,(1988) , 10.1093/NAR/16.23.10953
Andrew P. Feinberg, Bert Vogelstein, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity Analytical Biochemistry. ,vol. 132, pp. 6- 13 ,(1983) , 10.1016/0003-2697(83)90418-9
R. Saiki, S Scharf, F Faloona, K. Mullis, G. Horn, H. Erlich, N Arnheim, Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia Science. ,vol. 230, pp. 1350- 1354 ,(1985) , 10.1126/SCIENCE.2999980
Russell Higuchi, Cecilia H. von Beroldingen, George F. Sensabaugh, Henry A. Erlich, DNA typing from single hairs Nature. ,vol. 332, pp. 543- 546 ,(1988) , 10.1038/332543A0
V. Mehra, D. Sweetser, R. A. Young, Efficient mapping of protein antigenic determinants. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 7013- 7017 ,(1986) , 10.1073/PNAS.83.18.7013
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)