CELL-CYCLE AND PLOIDY ANALYSIS IN BONE MARROW AND LIVER CELLS OF RATS AFTER LONG-TERM CONSUMPTION OF IRRADIATED WHEAT

摘要: Abstract Rats were fed for 4 or 90 days either with 70% freshly irradiated wheat (0.25, 0.75 2.25 kGy) and 30% complementary feed a control diet. None of the parameters examined (food consumption, body weight, haematological analysis, histopathological inspection thymus, lung, liver, spleen kidney, DNA analysis bone marrow cells nuclei from liver by flow cytometry) showed any statistically significant association feeding regimen. Minor changes in ploidy cell cycling detectable (wheat-irradiation dose-dependent increase G2/M-phase up to 0.6%, decrease 8C 1.1% cells). From pattern alterations observed our study, radiolytic by-products irradiation spindle poison-like activity can be excluded. Polyploid do not accumulate within 90-day period. The minor effects on cycle are qualitatively comparable seen after food restriction animal studies. It is suggested that an altered composition fatty acids (the components most sensitive irradiation) responsible these marginal effects. Our findings may explain earlier Bhaskaram Sadasivan ( American Journal Clinical Nutrition 1975, 28, 130–135) who reported number polyploid lymphocytes malnourished children wheat. likely mechanisms such effect adaptive, constitutively regulated processes, similar those which respond restriction. concluded consumption does not, therefore, pose health risk humans.