Flow Cytometric Measurement of Cellular DNA Content Dispersion Induced by Mutagenic Treatment
作者： F. J. Otto , H. Oldiges , V. K. Jain
DOI: 10.1007/978-3-642-69334-2_4
关键词:
摘要: Recent improvements in flow-cytometric instrumentation and techniques enable us to make very precise measurements of distributions cellular DNA content populations suspended cells. Flow-cytometric are being used tumour research for the detection monitoring aneuploid cell lines [1, 47, 49] studying kinetics [15, 32, 33]. Similar could also be assessment cytogenetic damage induced by environmental agents like radiations or chemicals; example, measuring dispersion chromosomes whole cells a population [34–36, 39]. Such based on fact that chromosomal aberrations dysfunction mitotic apparatus due action mutagenic lead an unequal distribution amount daughter consequently increased variation population. This effect can observed as broadening G0/G1- G2+M-phase peaks quantitated. Work done along these will briefly reviewed following.
springer.com
core.ac.uk
sci-hub.st 参考文章(51)
G. Röhrborn, Assessment of Mutagenicity Tests Archives of Toxicology. ,vol. 4, pp. 3- 9 ,(1980) , 10.1007/978-3-642-67729-8_1
J. Schumann, H. Tilkorn, W. Göhde, F. Ehring, C. Straub, Zytogenetik maligner Melanome Springer, Berlin, Heidelberg. pp. 62- 66 ,(1981) , 10.1007/978-3-642-81671-0_21
P. Goetz, R.J. Šrám, J. Dohnalová, Relationship between experimental results in mammals and man: I. Cytogenetic analysis of bone marrow injury induced by a single dose of cyclophosphamide Mutation Research\/environmental Mutagenesis and Related Subjects. ,vol. 31, pp. 247- 254 ,(1975) , 10.1016/0165-1161(75)90007-2
G. Pinaev, D. Bandyopadhyay, O. Glebov, V. Shanbhag, G. Johansson, P.Å. Albertsson, Fractionation of chromosomes. I. A methodological study on the use of partition in aqueous two-phase systems and multiple sedimentation. Experimental Cell Research. ,vol. 124, pp. 191- 203 ,(1979) , 10.1016/0014-4827(79)90269-6
P. Goetz, R.J. Šrám, I. Kodýtková, J. Dohnalová, O. Dost'alová, J. Bártova, Relationship between experimental results in mammals and man. II. Cytogenetic analysis of bone-marrow cells after treatment of cytembena and cyclophosphamide- cytembena combination. Mutation Research. ,vol. 41, pp. 143- 152 ,(1975) , 10.1016/0027-5107(76)90084-1
Ashutosh Banerjee, William F. Benedict, Natarajan Venkatesan, Cyclophosphamide-induced oncogenic transformation, chromosomal breakage, and sister chromatid exchange following microsomal activation. Cancer Research. ,vol. 38, pp. 2922- 2924 ,(1978)
Herbert H. Spencer, Carlos E. Nasjleti, Chromosome Damage and Polyploidization Induced in Human Peripheral Leukocytes in Vivo and in Vitro with Nitrogen Mustard, 6-Mercaptopurine, and A-649 Cancer Research. ,vol. 26, pp. 2437- 2443 ,(1966)
Koichi I. Yamamoto, Yasumoto Kikuchi, Studies on micronuclei time response and on the effects of multiple treatments of mutagens on induction of micronuclei Mutation Research\/genetic Toxicology. ,vol. 90, pp. 163- 173 ,(1981) , 10.1016/0165-1218(81)90079-3
B.E. Matter, J. Grauwiler, Micronuclei in mouse bone-marrow cells. A simple in vivo model for the evaluation of drug-induced chromosomal aberrations. Mutation Research. ,vol. 23, pp. 239- 249 ,(1974) , 10.1016/0027-5107(74)90144-4
F. J. Otto, H. Oldiges, W. Göhde, V. K. Jain, Flow cytometric measurement of nuclear DNA content variations as a potential in vivo mutagenicity test Cytometry. ,vol. 2, pp. 189- 191 ,(1981) , 10.1002/CYTO.990020311