In Vitro Maturation of Human iPSC-Derived Neuroepithelial Cells Influences Transplant Survival in the Stroke-Injured Rat Brain
作者： Samantha L. Payne , Priya N. Anandakumaran , Balazs V. Varga , Cindi M. Morshead , Andras Nagy
DOI: 10.1089/TEN.TEA.2016.0515
关键词: Andrology 、 Viability assay 、 Regenerative medicine 、 Transplantation 、 Neuroepithelial cell 、 Stem cell 、 Population 、 Immunology 、 Biology 、 Regeneration (biology) 、 In vitro maturation
摘要: Stem cell transplantation is a promising strategy for brain tissue regeneration; yet, despite some success, survival following remains low. In this study, we demonstrate that viability enhanced by control over maturation of neuronal precursor cells, which are delivered in an injectable blend hyaluronan and methylcellulose. We selected three subpopulations human cells derived from cortically specified neuroepithelial stem (cNESC) population based on differences expression multipotent neuron-specific proteins: early-, mid-, late-differentiated neurons. These were transplanted into endothelin-1 stroke-injured rat their fate investigated 1 week later. Significantly, more found the after transplanting early- or mid- differentiated cNESCs compared to population. The mid-differentiated also had significantly β-III tubulin-positive than either populations. results suggest maturity has significant impact with intermediate differentiate
sci-hub.se 参考文章(39)
Travis Dailey, David J. Eve, Naoki Tajiri, Tsz Lau, Yusef Mosley, Harry van Loveren, Paul R. Sanberg, Yuji Kaneko, Cesario V. Borlongan, Different Sources of Stem Cells for Transplantation Therapy in Stroke Cell-Based Therapies in Stroke. pp. 29- 46 ,(2013) , 10.1007/978-3-7091-1175-8_3
Pouria Moshayedi, S. Thomas Carmichael, Biomaterials Application in Stem Cell Therapies for Stroke Springer International Publishing. pp. 213- 245 ,(2015) , 10.1007/978-3-319-15063-5_13
Stephen C. Noctor, Alexander C. Flint, Tamily A. Weissman, Ryan S. Dammerman, Arnold R. Kriegstein, Neurons derived from radial glial cells establish radial units in neocortex Nature. ,vol. 409, pp. 714- 720 ,(2001) , 10.1038/35055553
Brian G. Ballios, Michael J. Cooke, Laura Donaldson, Brenda L.K. Coles, Cindi M. Morshead, Derek van der Kooy, Molly S. Shoichet, A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation Stem cell reports. ,vol. 4, pp. 1031- 1045 ,(2015) , 10.1016/J.STEMCR.2015.04.008
Toru Yamashita, Hiromi Kawai, Fengfeng Tian, Yasuyuki Ohta, Koji Abe, Tumorigenic Development of Induced Pluripotent Stem Cells in Ischemic Mouse Brain Cell Transplantation. ,vol. 20, pp. 883- 891 ,(2011) , 10.3727/096368910X539092
Shawn B. Frost, Scott Barbay, Michael L. Mumert, Ann M. Stowe, Randolph J. Nudo, An animal model of capsular infarct: Endothelin-1 injections in the rat Behavioural Brain Research. ,vol. 169, pp. 206- 211 ,(2006) , 10.1016/J.BBR.2006.01.014
V WINDLE, A SZYMANSKA, S GRANTERBUTTON, C WHITE, R BUIST, J PEELING, D CORBETT, An analysis of four different methods of producing focal cerebral ischemia with endothelin-1 in the rat. Experimental Neurology. ,vol. 201, pp. 324- 334 ,(2006) , 10.1016/J.EXPNEUROL.2006.04.012
G Dziewczapolski, DC Lie, J Ray, FH Gage, CW Shults, Survival and differentiation of adult rat-derived neural progenitor cells transplanted to the striatum of hemiparkinsonian rats Experimental Neurology. ,vol. 183, pp. 653- 664 ,(2003) , 10.1016/S0014-4886(03)00212-7
Brian A. Aguado, Widya Mulyasasmita, James Su, Kyle J. Lampe, Sarah C. Heilshorn, Improving Viability of Stem Cells During Syringe Needle Flow Through the Design of Hydrogel Cell Carriers Tissue Engineering Part A. ,vol. 18, pp. 806- 815 ,(2012) , 10.1089/TEN.TEA.2011.0391
John Sharkey, Steven P. Butcher, Characterisation of an experimental model of stroke produced by intracerebral microinjection of endothelin-1 adjacent to the rat middle cerebral artery. Journal of Neuroscience Methods. ,vol. 60, pp. 125- 131 ,(1995) , 10.1016/0165-0270(95)00003-D