Effects of roughness, fibronectin and vitronectin on attachment, spreading, and proliferation of human osteoblast-like cells (Saos-2) on titanium surfaces.

摘要: The influence of surface roughness and the presence adhesion molecules in culture medium were studied regarding cell adhesion, shape, proliferation osteoblast-like cells grown on two types titanium disk. Type I disks acid etched type II sandblasted etched. Surface was determined by contact profilometry scanning electron microscopy. Chemical composition oxide thickness superficial layer established with energy dispersive X-ray spectrometry, spectroscopy for chemical analysis auger spectroscopy. Titanium release assessed inductively coupled plasma-optical emission spectrometry. Osteoblast-like (Saos-2) cultured both (1) standard conditions (DMEM supplemented fetal calf serum), (FCS), (2) alone alone), (3) fibronectin or vitronectin vitronectin). Cultures also performed monoclonal anti-integrin (β1, αv) to test involved binding surface. We found that sandblasting does not modify represents only 5–6% (in atom percentage) elements. Release increase from 24 72 hours. In absence FCS, fibronectin, vitronectin, appeared scanty packed clusters. On contrary, flattened large thin cytoplasmic expansions. addition anti β1 αv integrin subunit antibody decreased spreading cells, particularly fibronectin. Cell significantly higher plastic than disks, but increased rough smooth surfaces. These results indicate a high are critical elements spreading,