Exogenous polyamines promote osteogenic differentiation by reciprocally regulating osteogenic and adipogenic gene expression.

摘要: Polyamines are naturally occurring organic polycations that ubiquitous in all organisms, and essential for cell proliferation differentiation. Although polyamines involved various cellular processes, their roles stem differentiation relatively unexplored. In this study, we found exogenous polyamines, putrescine, spermidine, spermine, promoted osteogenic of human bone marrow-derived mesenchymal cells (hBMSCs) without inducing death or apoptosis. Alkaline phosphatase (ALP) activity the mRNA level genes, including Runx2, ALP, osteopontin, osteocalcin, were up-regulated by polyamines. When hBMSCs cultured at high density favoring adipocyte formation, resulted down-regulation adipogenic genes such as PPARγ, aP2, adipsin. Extracellular matrix mineralization, a marker osteoblast maturation, was enhanced presence while lipid accumulation, an indication differentiation, attenuated. Exogenous increased expression polyamine-modulated factor 1 (PMF-1) its downstream effector, spermidine/spermine N(1)-acetyltransferase (SSAT), ornithine decarboxylase (ODC), rate-limiting enzyme polyamine biosynthesis, suppressed. These results lead to possible connections between metabolism pathways. To summarize, study provides evidence involvement hBMSCs, is first demonstrate reciprocally regulated