Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast

摘要: In vivo optical microscopic imaging techniques have recently emerged as important tools for the study of neurobiological development and pathophysiology. particular, two-photon microscopy has proved to be a robust highly flexible method in scattering tissue. However, typically requires extrinsic dyes or contrast agents, depths are limited few hundred microns. Here we demonstrate Optical Coherence Microscopy (OCM) neuronal cell bodies cortical myelination up ~1.3 mm rat neocortex. Imaging does not require administration exogenous is achieved through intrinsic image processing alone. Furthermore, using OCM vivo, quantitative measurements properties (index refraction attenuation coefficient) cortex, correlate these with laminar cellular architecture determined from images. Lastly, show that enables direct visualization changes during depolarization may therefore provide novel markers viability.