Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1.

作者: F. Pfeiffer , S.C. Schuster , A. Broicher , M. Falb , P. Palm

DOI: 10.1016/J.YGENO.2008.01.001

关键词: CosmidStrain (chemistry)Insertion sequenceGeneticsGenomeHalobacterium salinarumComparative genomicsNucleic acid sequencePlasmidBiology

摘要: We report the sequence of Halobacterium salinarum strain R1 chromosome and its four megaplasmids. Our set protein-coding genes is supported by extensive proteomic homology data. The structures plasmids, which show three large-scale duplications (adding up to 100 kb), were unequivocally confirmed cosmid analysis. completely colinear virtually identical that NRC-1. Correlation plasmid sequences revealed 210 kb occurs only in R1. remaining 350 shows virtual identity two strains. Nevertheless, number overall structure plasmids are largely incompatible. Also, 20% protein differ despite near at DNA level. Finally, we genome-wide mobility data for insertion from conclude strains NRC-1 originate same natural isolate. This exemplifies evolution laboratory.

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