A thermosensitive hydrogel capable of releasing bFGF for enhanced differentiation of mesenchymal stem cell into cardiomyocyte-like cells under ischemic conditions.
作者： Zhenqing Li , Xiaolei Guo , Jianjun Guan
DOI: 10.1021/BM300574J
关键词: Cell biology 、 Cell survival 、 Growth factor 、 Nanotechnology 、 Trimethylene carbonate 、 Low oxygen 、 Infarcted heart 、 Acrylic acid 、 Mesenchymal stem cell 、 Chemistry
摘要: A thermosensitive hydrogel capable of differentiating mesenchymal stem cells (MSCs) into cardiomyocyte-like was synthesized. The based on N-isopropylacrylamide (NIPAAm), N-acryloxysuccinimide, acrylic acid, and hydroxyethyl methacrylate-poly(trimethylene carbonate). highly flexible at body temperature with breaking strain >1000% Young’s modulus 45 kPa. When MSCs were encapsulated in the cultured under normal culture conditions (10% FBS 21% O2), differentiated cells. However, differentiation retarded, even diminished, low nutrient oxygen conditions, which are typical infarcted heart. We hypothesized that enhancing MSC survival would restore differentiation. To enhance cell survival, a pro-survival growth factor (bFGF) loaded hydrogel. bFGF able to sustainedly release from for 21 days. Under a...
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sci-hub.se 参考文章(70)
Arlo N. McGinn, Hye Yeong Nam, Mei Ou, Norman Hu, Catherine M. Straub, James W. Yockman, David A. Bull, Sung Wan Kim, Bioreducible polymer-transfected skeletal myoblasts for VEGF delivery to acutely ischemic myocardium Biomaterials. ,vol. 32, pp. 942- 949 ,(2011) , 10.1016/J.BIOMATERIALS.2010.09.061
S. Faham, R. E. Hileman, J. R. Fromm, R. J. Linhardt, D. C. Rees, Heparin structure and interactions with basic fibroblast growth factor. Science. ,vol. 271, pp. 1116- 1120 ,(1996) , 10.1126/SCIENCE.271.5252.1116
Daisuke Asai, Donghua Xu, Wenge Liu, Felipe Garcia Quiroz, Daniel J. Callahan, Michael R. Zalutsky, Stephen L. Craig, Ashutosh Chilkoti, Protein polymer hydrogels by in situ, rapid and reversible self-gelation. Biomaterials. ,vol. 33, pp. 5451- 5458 ,(2012) , 10.1016/J.BIOMATERIALS.2012.03.083
Boonchoy Soontornworajit, Jing Zhou, Zhaoyang Zhang, Yong Wang, Aptamer-functionalized in situ injectable hydrogel for controlled protein release. Biomacromolecules. ,vol. 11, pp. 2724- 2730 ,(2010) , 10.1021/BM100774T
Zhenqing Li, Xiaolei Guo, Satoshi Matsushita, Jianjun Guan, Differentiation of cardiosphere-derived cells into a mature cardiac lineage using biodegradable poly(N-isopropylacrylamide) hydrogels Biomaterials. ,vol. 32, pp. 3220- 3232 ,(2011) , 10.1016/J.BIOMATERIALS.2011.01.050
Shi Zuo, W. Keith Jones, Hongxia Li, Zhisong He, Zeeshan Pasha, Yueting Yang, Yigang Wang, Guo-Chang Fan, Muhammad Ashraf, Meifeng Xu, Paracrine Effect of Wnt11-Overexpressing Mesenchymal Stem Cells on Ischemic Injury Stem Cells and Development. ,vol. 21, pp. 598- 608 ,(2012) , 10.1089/SCD.2011.0071
Ali Salim, Randall P. Nacamuli, Elise F. Morgan, Amato J. Giaccia, Michael T. Longaker, Transient changes in oxygen tension inhibit osteogenic differentiation and runx2 expression in osteoblasts Journal of Biological Chemistry. ,vol. 279, pp. 40007- 40016 ,(2004) , 10.1074/JBC.M403715200
Gaël Y. Rochefort, Bruno Delorme, Adriana Lopez, Olivier Hérault, Pierre Bonnet, Pierre Charbord, Véronique Eder, Jorge Domenech, Multipotential mesenchymal stem cells are mobilized into peripheral blood by hypoxia. Stem Cells. ,vol. 24, pp. 2202- 2208 ,(2006) , 10.1634/STEMCELLS.2006-0164
David M. Hoganson, Elisabeth M. O’Doherty, Gwen E. Owens, Dina O. Harilal, Scott M. Goldman, Chris M. Bowley, Craig M. Neville, Russell T. Kronengold, Joseph P. Vacanti, The retention of extracellular matrix proteins and angiogenic and mitogenic cytokines in a decellularized porcine dermis. Biomaterials. ,vol. 31, pp. 6730- 6737 ,(2010) , 10.1016/J.BIOMATERIALS.2010.05.019
Jie Deng, Yaling Han, Chenhui Yan, XIaoxiang Tian, Jie Tao, Jian Kang, Shaohua Li, Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt Apoptosis. ,vol. 15, pp. 463- 473 ,(2010) , 10.1007/S10495-009-0434-7