Paracrine Effect of Wnt11-Overexpressing Mesenchymal Stem Cells on Ischemic Injury
作者： Shi Zuo , W. Keith Jones , Hongxia Li , Zhisong He , Zeeshan Pasha
关键词: Biology 、 Myocardial infarction 、 Programmed cell death 、 Cardiac function curve 、 Pathology 、 Immunology 、 Transplantation 、 Cardioprotection 、 Mesenchymal stem cell 、 Masson's trichrome stain 、 Paracrine signalling
摘要: Our previous studies have suggested that transduction of Wnt11 directly increases bone marrow-derived mesenchymal stem cells (MSCs) differentiation into cardiac phenotypes. In this study, we investigated whether enhances MSC-mediated cardioprotection via paracrine fashion after acute ischemia. MSCs were harvested from male rat marrow and transduced with (MSCWnt11). An myocardial infarction model in rats was developed by ligation the left anterior descending coronary artery. MSCWnt11 transplanted peri-infarct region infarction. To mimic ischemic injury, cultured cardiomyocytes (CMs) isolated neonatal ventricles exposed to hypoxia. ELISA indicated release (3.45-fold) as well transforming growth factor-β2 (TGFβ2) (1.5-fold) significantly increased compared control MSC (MSCNull). Hypoxia-induced apoptosis cell death reduced when CM co-cultured a dual chamber system. The protection mediated mimicked treating conditioned medium obtained abrogated Wnt11- TGFβ2 neutralizing antibodies. Further, animals receiving showed significant improvement contractile function assessed echocardiography. Masson trichrome TUNEL staining reduction infarct size MSCWnt11-treated animals. Transplantation improved function. other factors is more likely responsible for native at risk.
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