Human dendritic cells transfected with amplified MUC1 mRNA stimulate cytotoxic T lymphocyte responses against pancreatic cancer in vitro.
作者： Jiang Chen , Hong‐Yu Li , Di Wang , Jia‐Jun Zhao , Xiao‐Zhong Guo
DOI: 10.1111/J.1440-1746.2011.06778.X
关键词: Biology 、 Molecular biology 、 Immunotherapy 、 CTL* 、 Major histocompatibility complex 、 Antigen presentation 、 Antigen 、 MUC1 、 Cancer research 、 Cytotoxic T cell 、 MHC class I
摘要: Background and Aim: Mucin (MUC) 1 is an epithelial cell glycoprotein that aberrantly overexpressed in many adenocarcinomas, including pancreatic cancer (PC), providing ideal tumor-associated antigen target for immunotherapy. In this study, we investigated whether the cytotoxic T lymphocytes (CTLs) induced by dendritic cells (DCs) transfected with amplified MUC1 mRNA could respond against PC vitro. Methods: Amplified encoding were into DCs using electroporation optimized setting expression evaluated quantitative real-time polymerase chain reaction Western blot. The specific CTL responses measured standard chromium 51 (51Cr)-release assays interferon-γ release assay. Results: Dendritic be efficiently. remarkably effective stimulating MUC1-specific vitro. function of CTLs, mRNA-transfected DCs, was restricted major histocompatibility complex (MHC) class I presentation. Conclusion: stimulated only recognize lyse HLA-A2+/MUC1+ other under restriction MHC I-specific presentation, a preclinical rationale as structure immunotherapeutic strategies PC.
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