Circular probe amplification (cpa) using energy-transfer primers
作者： David C. Thomas
DOI:
关键词: Energy transfer 、 Nucleic acid 、 Chemistry 、 Molecular biology 、 Combinatorial chemistry
摘要: The present invention provides methods and kits for the rapid exponential amplification of nucleic acid molecules using a padlock probe. improves upon existing amplifying probes by eliminating or delaying appearance artifact products that cause false positive results, also increase sensitivity speed assay. Further provided are primers containing non-informative base analogs.
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