Method to produce single stranded dna of defined length and sequence and dna probes produced thereby
作者： Sujatha Krishnakumar , Ronald W. Davis , Michael Mindrinos
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摘要: A method for producing a single stranded DNA (ssDNA) molecule of defined length and sequence is disclosed. This enables the preparation of, inter alia, probes greater than can be chemically synthesized. The starts with double molecule, such as genomic, (dsDNA) from any organism. fragment starting amplified by specific primers engineered to introduce cleavage sites on either side desired sequence. Cleavage steps amplified, are combined phosphate removal step, thereby creating construct that digested an exonuclease without damage ssDNA. Probes, which hybridize large gaps between ends probes, also
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