Hemisphere-dependent endocannabinoid system activity in prefrontal cortex and hippocampus of the Flinders Sensitive Line rodent model of depression.
作者： C. Kirkedal , B. Elfving , H.K. Müller , F.A. Moreira , L. Bindila
DOI: 10.1016/J.NEUINT.2019.01.023
关键词: Internal medicine 、 Monoacylglycerol lipase 、 Endocannabinoid system 、 Fatty acid amide hydrolase 、 Anandamide 、 Endocrinology 、 Cannabinoid 、 Hippocampus 、 Chemistry 、 Receptor 、 Prefrontal cortex
摘要: Altered endocannabinoid (eCB) signalling is suggested as an important contributor to the pathophysiology of depression. To further elucidate this, we conducted a study using genetic rat model depression, Flinders Sensitive Line (FSL), and their controls, Resistant (FRL) rats. Plasma, right left prefrontal cortex, hippocampus were isolated from FSL FRL We analyzed each region for eCB anandamide (AEA) 2-arachidonoylglycerol (2-AG) levels by liquid chromatography/multiple reaction monitoring (LC/MRM), mRNA protein cannabinoid type 1 receptor (CB1R), fatty acid amide hydrolase (FAAH) monoacyl glycerol lipase (MAGL) real time qPCR Western blotting. Content 2-AG was lower in side cortex rats compared Inversely, AEA higher than hippocampus. In plasma, increased decreased. Cannabinoid (Cnr1), Faah Magl prominently decreased Protein expression CB1R FAAH summary, our data suggest rats, which could contribute depressive-like behaviour. Interestingly, altered system activity appear be hemisphere-specific limbic regions. Our support existing literature showed this particular animal
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