Predictive observation-based endpoint criteria for mice receiving total body irradiation.
作者： Jeffrey D Fortman , Elizabeth A Nunamaker , Alexander V Lyubimov , James E Artwohl , Robert J Anderson
DOI:
关键词: Internal medicine 、 Total body 、 Whole body irradiation 、 Total body irradiation 、 Surgery 、 Acute Radiation Syndrome 、 Retrospective analysis 、 Scoring system 、 Body posture 、 Medicine
摘要: Total body irradiation of mice is a commonly used research technique; however, humane endpoints have not been clearly identified. This situation has led to the inconsistent use various endpoints, including death. To address this issue, we refined cageside observation-based scoring system specifically for receiving total irradiated. Male and female C57BL/6 (age, 8 wk) received 1 3 doses radiation from 2 different sources were observed progression clinical signs. All scored individually by using observations their posture (score, 0 3), eye appearance (0 activity level 3). Retrospective analysis observation score data indicated that death could be predicted accurately with scores 7 or greater, consistent between observers. can increase endpoint criteria in murine studies ultimately improve animal welfare.
参考文章(31)
David W. Kennedy, Janis L. Abkowitz, Kinetics of Central Nervous System Microglial and Macrophage Engraftment: Analysis Using a Transgenic Bone Marrow Transplantation Model Blood. ,vol. 90, pp. 986- 993 ,(1997) , 10.1182/BLOOD.V90.3.986
Jeffrey D Fortman, Elizabeth A Nunamaker, James E Artwohl, Robert J Anderson, Endpoint refinement for total body irradiation of C57BL/6 mice. Comparative Medicine. ,vol. 63, pp. 22- 28 ,(2013)
RJ Jones, P Celano, SJ Sharkis, LL Sensenbrenner, Two Phases of Engraftment Established by Serial Bone Marrow Transplantation in Mice Blood. ,vol. 73, pp. 397- 401 ,(1989) , 10.1182/BLOOD.V73.2.397.BLOODJOURNAL732397
Krinon D Moccia, Cara H Olsen, Michael R Landauer, Jennifer M Mitchell, Evaluation of hydration and nutritional gels as supportive care after total-body irradiation in mice (Mus musculus). Journal of The American Association for Laboratory Animal Science. ,vol. 49, pp. 323- 328 ,(2010)
D. Morton, P. Griffiths, Guidelines on the recognition of pain, distress and discomfort in experimental animals and an hypothesis for assessment. Veterinary Record. ,vol. 116, pp. 431- 436 ,(1985) , 10.1136/VR.116.16.431
Robert Clarke, Issues in experimental design and endpoint analysis in the study of experimental cytotoxic agents in vivo in breast cancer and other models. Breast Cancer Research and Treatment. ,vol. 46, pp. 255- 278 ,(1997) , 10.1023/A:1005938428456
K. A. Jackson, T. Mi, M. A. Goodell, Hematopoietic potential of stem cells isolated from murine skeletal muscle. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 14482- 14486 ,(1999) , 10.1073/PNAS.96.25.14482
Diane S. Krause, Neil D. Theise, Michael I. Collector, Octavian Henegariu, Sonya Hwang, Rebekah Gardner, Sara Neutzel, Saul J. Sharkis, Multi-Organ, Multi-Lineage Engraftment by a Single Bone Marrow-Derived Stem Cell Cell. ,vol. 105, pp. 369- 377 ,(2001) , 10.1016/S0092-8674(01)00328-2
Robert F. Kallman, Giovanni Silini, Recuperation from Lethal Injury by Whole-Body Irradiation: I. Kinetic Aspects and the Relationship with Conditioning Dose in C57BL Mice Radiation Research. ,vol. 22, pp. 622- ,(1964) , 10.2307/3571544
E.L. Travis, L.J. Peters, J. McNeill, H.D. Thames, C. Karolis, Effect of dose-rate on total body irradiation: lethality and pathologic findings. Radiotherapy and Oncology. ,vol. 4, pp. 341- 351 ,(1985) , 10.1016/S0167-8140(85)80122-5