Significance of c-erb5-2 amplification and DNA aneuploidy. Analysis in 78 patients with node-negative breast cancer

摘要: BACKGROUND Amplification of the c-erbB-2 protooncogene and DNA aneuploidy have been reported to correlate with poor patient prognosis in human breast cancer. Several studies investigated prognostic value these two factors heterogeneous populations patients node-positive node-negative disease. This study evaluated, on a series disease, whether proto-oncogene amplification cellular content could identify subset who, without adjuvant therapy, are destined experience relapse. METHODS Paraffin-embedded tissues 78 were evaluated for using flow cytometric analysis. was determined same group slot-blot hybridization. The majority matched control subjects following five clinicopathologic criteria: size primary tumor, menopausal status, estrogen receptor, anniversary year initial treatment, age at treatment. Long-term follow-up (5-16 years) available each patient, none whom received any form therapy. RESULTS presence an abnormal stemline found 47% (37 78) tissue specimens, whereas only 10% (8 tumors expressed from 3-fold 22-fold amplification. Combined occurred small (n = 4), all experienced four remaining having excessive gene copy numbers had diploid distribution. CONCLUSIONS results indicate that overexpress variable amounts ploidy analysis provide limited predictive information relapse Although combination may be predictor number patients, neither measurement alone is effective identifying increased risk recurrence