Gold-facilitated in situ hybridization: a bright-field autometallographic alternative to fluorescence in situ hybridization for detection of Her-2/neu gene amplification.

作者: Raymond Tubbs , James Pettay , Marek Skacel , Richard Powell , Mark Stoler

DOI: 10.1016/S0002-9440(10)61106-6

关键词: MicroscopyBright-field microscopyFluorescence in situ hybridizationIn situGene duplicationIn situ hybridizationImmunohistochemistryBiologyGene expressionMolecular biology

摘要: … The method is summarized in Figure 1. Unstained 4-μm paraffin sections on electrostatically … Gold ions in solution are catalytically deposited onto the Nanogold particle as metallic gold (…

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