Efficient Epstein-Barr Virus Progeny Production Mediated by Cancer-Derived LMP1 and Virally-Encoded microRNAs

摘要: Epstein-Barr virus (EBV) genomes, particularly their latent genes, are heterogeneous among strains. The heterogeneity of EBV-encoded membrane protein 1 (LMP1) raises the question whether there functional differences between LMP1 expressed by cancer-associated EBV and that non-cancerous Here, we used bacterial artificial chromosome (BAC)-cloned genomes retaining all virally encoded microRNA (miRNA) genes to investigate functions cancer-derived in context genome. HEK293 cells were stably transfected with EBV-BAC clone DNAs encoding either nasopharyngeal carcinoma (NPC)-derived CAO-LMP1 (LMP1CAO) or from a prototype B95-8 strain (LMP1B95-8). When an DNA LMP1CAO was into cells, it generated many more stable transformants than control LMP1B95-8. Furthermore, exhibited highly efficient production progeny virus. Importantly, deletion clustered viral miRNA compromised ability produce viruses. These results indicate miRNAs together necessary for virus, resulting increase efficiency contributes EBV-mediated epithelial carcinogenesis.