HPLC quantification of the HIV-1 protease inhibitor saquinavir in brain and testis of mice.
作者： Koteshwara Mudigonda , Raju Jukanti , Shashank S. Apte , Devender R. Ajjala , Wishu Shrivastava
DOI: 10.1002/BMC.631
关键词: HIV-1 protease 、 Lower limit 、 Chemistry 、 Chromatography 、 Drug delivery 、 Saquinavir 、 Hplc quantification 、 Hplc method 、 Tissue distribution 、 High-performance liquid chromatography
摘要: A rapid, reliable HPLC method with UV detection (240 nm) was developed and validated for quantitation of saquinavir in mice brain testis. Saquinavir the internal standard were isolated from homogenized tissue matrices using liquid-liquid extraction procedure then analyzed an isocratic mobile phase by reversed-phase liquid chromatography. The lower limit quantification 50 ng/g both linear dynamic range 50-5000 testis established. This between-batch precision 0.5-4.4 1.5-5.5% testis, respectively. accuracy 94.7-105.9% 97.5-105.0% present applied distribution studies novel drug delivery systems mice.
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