Ginsenoside RG1 enhances the paracrine effects of bone marrow-derived mesenchymal stem cells on radiation induced intestinal injury.
作者： Yujun Luo , Beibei Wang , Jianhua Liu , Faxin Ma , Dongling Luo
关键词: Mesenchymal stem cell 、 Angiogenesis 、 Downregulation and upregulation 、 Cancer research 、 Bone marrow 、 Paracrine signalling 、 Stem cell 、 Chemistry 、 Inflammation 、 Regeneration (biology)
摘要: Content and aims: Ginsenoside RG1 (RG1) is thought to enhance proliferation differentiation of stem cell, however, its role on paracrine efficacy cell remains unclear. Here we examined if how enhances the effects bone marrow-derived mesenchymal cells (BM-MSCs) radiation induced intestinal injury (RIII). Method Irradiated rats randomly received intraperitoneal injection conditioned medium (CM) derived from non-activated BM-MSCs (MSC-CM) or pre-activated by RG-1 (RG1-MSC-CM). Intestinal samples were collected, followed evaluation histological functional change, apoptosis, proliferation, inflammation, angiogenesis regeneration. The heme oxygenase-1 (HO-1) investigated using HO-1 inhibitor siRNA. Result enhanced partially through upregulation HO-1. RG1-MSC-CM rather than MSC-CM significantly improved survival damage irradiated via improvement proliferation/apoptosis, regeneration in a dependent mechanism. mechanism for superior related higher release two pivotal cytokines VEGF IL-6. Conclusion Our study revealed that RIII, providing novel method maximizing potential MSCs.
sci-hub.st 参考文章(65)
Ismail H. Mallick, Marc C. Winslet, Alexander M. Seifalian, Pyrrolidine dithiocarbamate protects the small bowel from warm ischaemia/reperfusion injury of the intestine: the role of haem oxygenase Clinical Science. ,vol. 111, pp. 373- 380 ,(2006) , 10.1042/CS20060119
Denis Angoulvant, Fabrice Ivanes, René Ferrera, Phoebe G. Matthews, Serge Nataf, Michel Ovize, Mesenchymal stem cell conditioned media attenuates in vitro and ex vivo myocardial reperfusion injury. Journal of Heart and Lung Transplantation. ,vol. 30, pp. 95- 102 ,(2011) , 10.1016/J.HEALUN.2010.08.023
Cui Chen, Xin-yi Mu, Yue Zhou, Ke Shun, Shan Geng, Jun Liu, Jian-wei Wang, Jie Chen, Tin-yu Li, Ya-ping Wang, Ginsenoside Rg1 enhances the resistance of hematopoietic stem/progenitor cells to radiation-induced aging in mice Acta Pharmacologica Sinica. ,vol. 35, pp. 143- 150 ,(2014) , 10.1038/APS.2013.136
Kyrylo Tron, Ruslan Novosyadlyy, Jozsef Dudas, Anatoly Samoylenko, Thomas Kietzmann, Giuliano Ramadori, Upregulation of heme oxygenase-1 gene by turpentine oil-induced localized inflammation: involvement of interleukin-6 Laboratory Investigation. ,vol. 85, pp. 376- 387 ,(2005) , 10.1038/LABINVEST.3700228
David Zisa, Arsalan Shabbir, Gen Suzuki, Techung Lee, Vascular endothelial growth factor (VEGF) as a key therapeutic trophic factor in bone marrow mesenchymal stem cell-mediated cardiac repair Biochemical and Biophysical Research Communications. ,vol. 390, pp. 834- 838 ,(2009) , 10.1016/J.BBRC.2009.10.058
Jochen Müller-Ehmsen, Peter Whittaker, Robert A. Kloner, Joan S. Dow, Tsuyoshi Sakoda, Tiffany I. Long, Peter W. Laird, Larry Kedes, Survival and development of neonatal rat cardiomyocytes transplanted into adult myocardium. Journal of Molecular and Cellular Cardiology. ,vol. 34, pp. 107- 116 ,(2002) , 10.1006/JMCC.2001.1491
Elena A Komarova, Konstantin Christov, Alexander I Faerman, Andrei V Gudkov, Different impact of p53 and p21 on the radiation response of mouse tissues Oncogene. ,vol. 19, pp. 3791- 3798 ,(2000) , 10.1038/SJ.ONC.1203717
Daan van Poll, Biju Parekkadan, Cheul H. Cho, François Berthiaume, Yaakov Nahmias, Arno W. Tilles, Martin L. Yarmush, Mesenchymal stem cell-derived molecules directly modulate hepatocellular death and regeneration in vitro and in vivo. Hepatology. ,vol. 47, pp. 1634- 1643 ,(2008) , 10.1002/HEP.22236
Wei Eric Wang, Dezhong Yang, Liangpeng Li, Wei Wang, Yulan Peng, Caiyu Chen, Peng Chen, Xuewei Xia, Hongyong Wang, Jiahui Jiang, Qiao Liao, Yuan Li, Ganfeng Xie, Haiyun Huang, Yanli Guo, Linda Ye, Dayue Darrel Duan, Xiongwen Chen, Steven R. Houser, Chunyu Zeng, Prolyl Hydroxylase Domain Protein 2 Silencing Enhances the Survival and Paracrine Function of Transplanted Adipose-Derived Stem Cells in Infarcted Myocardium Circulation Research. ,vol. 113, pp. 288- 300 ,(2013) , 10.1161/CIRCRESAHA.113.300929
Shih-Chieh Hung, Radhika R. Pochampally, Sy-Chi Chen, Shu-Ching Hsu, Darwin J. Prockop, Angiogenic Effects of Human Multipotent Stromal Cell Conditioned Medium Activate the PI3K-Akt Pathway in Hypoxic Endothelial Cells to Inhibit Apoptosis, Increase Survival, and Stimulate Angiogenesis STEM CELLS. ,vol. 25, pp. 2363- 2370 ,(2007) , 10.1634/STEMCELLS.2006-0686