Glutamate induces de novo growth of functional spines in developing cortex

摘要: Mature cortical pyramidal neurons receive excitatory inputs onto small protrusions emanating from their dendrites called spines. Spines undergo activity-dependent remodelling, stabilization and pruning during development, similar structural changes can be triggered by learning in sensory experiences. However, the biochemical triggers mechanisms of de novo spine formation developing brain functional significance new spines to neuronal connectivity are largely unknown. Here we develop an approach induce monitor real time using combined two-photon laser-scanning microscopy laser uncaging glutamate. Our data demonstrate that, mouse layer 2/3 neurons, glutamate is sufficient trigger growth dendrite shaft a location-specific manner. We find that glutamate-induced spinogenesis requires opening NMDARs (N-methyl-D-aspartate-type receptors) activation protein kinase A (PKA) but independent calcium-calmodulin-dependent II (CaMKII) tyrosine receptor B (TrkB) receptors. Furthermore, newly formed express receptors rapidly such they transduce presynaptic activity into postsynaptic signals. Together, our early neural shaped spatially precise manner nascent functionally incorporated circuits.