A rapid SNAP-tag fluorogenic probe based on an environment-sensitive fluorophore for no-wash live cell imaging.
作者： Tao-Kai Liu , Pei-Ying Hsieh , Yu-De Zhuang , Chi-Yang Hsia , Chi-Ling Huang
DOI: 10.1021/CB500502N
关键词: Nanotechnology 、 Molecular imaging 、 SNAP-tag 、 Biophysics 、 Fluorophore 、 Live cell imaging 、 Fluorescence 、 Intracellular 、 Cell Surface Proteins 、 Molecular probe 、 Chemistry
摘要: … The probe exhibits dramatic fluorescence turn-on of 280-fold upon being labeled to SNAP-tag. The major advantages of our fluorogenic probe are the dramatic fluorescence turn-on, …
acs.org
nctu.edu.tw参考文章(31)
Katharina Stöhr, Daniel Siegberg, Tanja Ehrhard, Konstantinos Lymperopoulos, Simin Öz, Sonja Schulmeister, Andrea C. Pfeifer, Julie Bachmann, Ursula Klingmüller, Victor Sourjik, Dirk-Peter Herten, Quenched substrates for live-cell labeling of SNAP-tagged fusion proteins with improved fluorescent background Analytical Chemistry. ,vol. 82, pp. 8186- 8193 ,(2010) , 10.1021/AC101521Y
Chaoran Jing, Virginia W. Cornish, A Fluorogenic TMP-Tag for High Signal-to-Background Intracellular Live Cell Imaging ACS Chemical Biology. ,vol. 8, pp. 1704- 1712 ,(2013) , 10.1021/CB300657R
A. Keppler, H. Pick, C. Arrivoli, H. Vogel, K. Johnsson, Labeling of fusion proteins with synthetic fluorophores in live cells Proceedings of the National Academy of Sciences of the United States of America. ,vol. 101, pp. 9955- 9959 ,(2004) , 10.1073/PNAS.0401923101
Stephen R. Adams, Robert E. Campbell, Larry A. Gross, Brent R. Martin, Grant K. Walkup, Yong Yao, Juan Llopis, Roger Y. Tsien, New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications. Journal of the American Chemical Society. ,vol. 124, pp. 6063- 6076 ,(2002) , 10.1021/JA017687N
Christopher Chidley, Hirohito Haruki, Miriam Grønlund Pedersen, Evelyne Muller, Kai Johnsson, A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis Nature Chemical Biology. ,vol. 7, pp. 375- 383 ,(2011) , 10.1038/NCHEMBIO.557
Yuichiro Hori, Hideki Ueno, Shin Mizukami, Kazuya Kikuchi, Photoactive yellow protein-based protein labeling system with turn-on fluorescence intensity. Journal of the American Chemical Society. ,vol. 131, pp. 16610- 16611 ,(2009) , 10.1021/JA904800K
Antje Keppler, Susanne Gendreizig, Thomas Gronemeyer, Horst Pick, Horst Vogel, Kai Johnsson, A general method for the covalent labeling of fusion proteins with small molecules in vivo Nature Biotechnology. ,vol. 21, pp. 86- 89 ,(2003) , 10.1038/NBT765
Nils Johnsson, Kai Johnsson, Chemical tools for biomolecular imaging. ACS Chemical Biology. ,vol. 2, pp. 31- 38 ,(2007) , 10.1021/CB6003977
Arnaud Gautier, Eiji Nakata, Gražvydas Lukinavičius, Kui-Thong Tan, Kai Johnsson, Selective cross-linking of interacting proteins using self-labeling tags. Journal of the American Chemical Society. ,vol. 131, pp. 17954- 17962 ,(2009) , 10.1021/JA907818Q
Carsten Hoffmann, Guido Gaietta, Alexander Zürn, Stephen R Adams, Sonia Terrillon, Mark H Ellisman, Roger Y Tsien, Martin J Lohse, Fluorescent labeling of tetracysteine-tagged proteins in intact cells Nature Protocols. ,vol. 5, pp. 1666- 1677 ,(2010) , 10.1038/NPROT.2010.129