Near-infrared fluorescence activation probes based on disassembly-induced emission cyanine dye

摘要: Currently most of the fluorogenic probes are designed for detection enzymes which work by converting non-fluorescence substrate into fluorescence product via an enzymatic reaction. On other hand, design non-enzymatic proteins remains a great challenge. Herein, we report general strategy to create near-IR probes, where small molecule ligand is conjugated novel γ-phenyl-substituted Cy5 fluorophore, selective through process. Detail mechanistic studies reveal that self-assemble form fluorescence-quenched J-type aggregate. In presence target analyte, bright in region emitted recognition-induced disassembly probe This fluorophore unique self-assembly/disassembly dye as it gives remarkable enhancement. Based on same design, three different were constructed and one them was applied no-wash imaging tumor cells hypoxia-induced cancer-specific biomarker, transmembrane-type carbonic anhydrase IX.