IL‑22 expression is increased variedly in the initial phase, onset and chronic phase of a pristane‑induced arthritis rat model

摘要: The aim of the present study was to investigate expression pattern T helper (Th) 17 and Th22 cell-related factors in a pristane‑induced arthritis (PIA) rat model. PIA rats were divided into initial phase group [day (D) 6 post‑pristane injection], onset clinical (D12), acute (D26) chronic (D70). Rats injected with saline alone used as control group. mRNA levels interleukin (IL)‑17A, IL‑17F, interferon (IFN)‑γ, IL‑22, IL‑22 receptor (R) 1, binding protein (BP) RAR‑related orphan α examined spleen and/or synovium various phases by reverse transcription‑quantitative polymerase chain reaction analysis. results demonstrated that, spleen, exhibited an increasing trend both disease, while ratio IL‑22R1/IL‑22BP increased phases, compared During phase, IL‑17F IFN‑γ significantly IL‑17A tendency synovium, In IL‑22R1 synovium. addition, immunohistochemistry analysis evaluate IL‑17A, IL‑21, ankle joints D26 rats. mainly expressed infiltrated inflammatory cells IL‑21 articular chondrocyte proliferative zone. proliferating synovial cells. conclusion, Th17 Th22‑related factor varied different disease progression tissues It is thought that may serve important role pathological process PIA, particularly fluctuation phase. Therefore, it be candidate molecule for treatment rheumatoid arthritis.