A cryptic role of a glycolytic–gluconeogenic enzyme (aldolase) in amino acid transporter turnover in Aspergillus nidulans

摘要: Abstract In Aspergillus nidulans the fbaA1013 mutation results in reduced or total loss of growth on glycolytic and gluconeogenic carbon sources, respectively. It also negatively affects several amino acids (including l -proline, -glutamate -aspartate) that fungus can use as nitrogen source sources. Complementation using an A. genomic library resulted cloning fbaA gene, which encodes a putative fructose 1,6-biphosphate aldolase (FBA), enzyme involved both glycolysis gluconeogenesis. The is chromosome rearrangement 5′ regulatory region gene resulting transcription response to sources essential for growth. A functional FbaA protein necessary plasma membrane localization AgtA acidic acid ( -glutamate/ transporter, targeting presumably subsequent degradation vacuole. Our support novel role is, involvement regulation transporters.