A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay
作者： Yun-ping Zhang , J.K Uyemoto , B.C Kirkpatrick
DOI: 10.1016/S0166-0934(97)00190-0
关键词: RNA 、 Nucleic acid 、 Agarose gel electrophoresis 、 Viroid 、 Biology 、 Molecular biology 、 Peach latent mosaic viroid 、 DNA 、 Chromatography 、 Polymerase chain reaction 、 Xylella fastidiosa
摘要: … phytoplasma DNA and PLMVd RNA and easily detected by PCR or RT-PCR (Zhang, unpublished data). We used these procedures to extract nucleic acids from various woody hosts, …
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sci-hub.se 参考文章(16)
A.M. Shamloul, A. Minafra, A. Hadidi, H.E. Waterworth, L. Giunchedi, E.K. Allam, Peach latent mosaic viroid: nucleotide sequence of an Italian isolate, sensitive detection using RT-PCR and geographic distribution. XVIth International Symposium on fruit tree virus diseases, 27 June - 2 July, 1994, Rome, Italy.. pp. 522- 530 ,(1995) , 10.17660/ACTAHORTIC.1995.386.75
M.J. Dallwitz, L. Watson, K. Crabtree, A.A. Brunt, A.J. Gibbs, Viruses of plants :descriptions and lists from the VIDE database : CAB International. ,(1996)
B. Schneider, E. Seemüller, Presence of Two Sets of Ribosomal Genes in Phytopathogenic Mollicutes Applied and Environmental Microbiology. ,vol. 60, pp. 3409- 3412 ,(1994) , 10.1128/AEM.60.9.3409-3412.1994
TJ Morris, JA Dodds, Isolation and Analysis of Double-Stranded RNA from Virus-Infected Plant and Fungal Tissue Phytopathology. ,vol. 69, pp. 854- 858 ,(1979) , 10.1094/PHYTO-69-854
Sujun Deng, Chuji Hiruki, Amplification of 16S rRNA genes from culturable and nonculturable mollicutes Journal of Microbiological Methods. ,vol. 14, pp. 53- 61 ,(1991) , 10.1016/0167-7012(91)90007-D
Donald J. MacKenzie, Morven A. McLean, Srima Mukerji, Margaret Green, Improved RNA Extraction from Woody Plants for the Detection of Viral Pathogens by Reverse Transcription-Polymerase Chain Reaction. Plant Disease. ,vol. 81, pp. 222- 226 ,(1997) , 10.1094/PDIS.1997.81.2.222
Hong Wang, Meiqing Qi, Adrian J. Cutler, A simple method of preparing plant samples for PCR Nucleic Acids Research. ,vol. 21, pp. 4153- 4154 ,(1993) , 10.1093/NAR/21.17.4153
J.S. Semancik, Separation of viroid RNAs by cellulose chromatography indicating conformational distinctions Virology. ,vol. 155, pp. 39- 45 ,(1986) , 10.1016/0042-6822(86)90166-2
Laurene Levy, Ing-Ming Lee, Ahmed Hadidi, Simple and rapid preparation of infected plant tissue extracts for PCR amplification of virus, viroid, and MLO nucleic acids. Journal of Virological Methods. ,vol. 49, pp. 295- 304 ,(1994) , 10.1016/0166-0934(94)90144-9
G. V. Minsavage, Development of a Polymerase Chain Reaction Protocol for Detection ofXylella fastidiosain Plant Tissue Phytopathology. ,vol. 84, pp. 456- 461 ,(1994) , 10.1094/PHYTO-84-456