Adenosine deaminase: characterization of the molecular heterogeneity of the enzyme in human tissue.

摘要: Adenosine deaminase (ADA) catalyzes the irreversible hydrolytic deamination of adenosine to produce inosine and ammonia. In human tissue this activity exhibits considerable molecular heterogeneity in that “tissue specific isoenzymes” have been demonstrated with either starch gel electrophoresis (1–3) or filtration (3,4,7). The weights multiple forms apparent varied from 30,000 47,000 (3–7) 230,000 440,000 Although basis for has obscure, definition characteristics residual ADA tissues patients severe combined immunodeficiency deficiency (8,9), it proposed is result post-translational modification a single locus product (9). For hypothesis be valid, interconvertibility between should demonstrated. study we examined nature demonstrate evident are interconvertible.