Prognostic Implications of Proliferative Activity and DNA Aneuploidy in Astler-Coller Dukes Stage C Colonic Adenocarcinomas
作者： Tariq Murad , Joan S. Chmiel , Dante G. Scarpelli , Linda Poggensee , Brenda L. Eriksen
DOI:
关键词: Transverse colon 、 Lymph 、 Gastroenterology 、 Dukes stage 、 Proportional hazards model 、 Flow cytometry 、 Multivariate analysis 、 Adenocarcinoma 、 Biology 、 Internal medicine 、 Histopathology 、 Pathology
摘要: Paraffin-embedded surgical specimens from 69 patients who underwent resections of otherwise untreated Dukes stage C adenocarcinoma the colon were examined for proliferative activity, DNA aneuploidy, index, and proportion aneuploid cells by flow cytometry. Results correlated to clinical characteristics overall survival times. tumors identified in 60 cases (87%), diploid 9 (13%). The mean S-phase fraction all was 17.6%, with a standard deviation (SD) 7.8. In univariate statistical analysis, younger patient age, lower tumor index less than or equal 1.2, presence only 1-4 lymph nodes involvement found be significant predictors improved survival. multivariate Cox regression low location right transverse independent increased When activity stratified into statistically defined subgroups, (S-phase - 0.5 SD) had significantly longer moderate value greater SD +0.5 high SD). These results suggest that carcinoma may an important biological factor determining prognosis.
aacrjournals.org参考文章(24)
Tariq Murad, Alan L. Epstein, Charles V. Clevenger, Dante G. Scarpelli, Rosalind K. Endow, Kenneth D. Bauer, Simultaneous nuclear antigen and DNA content quantitation using paraffin-embedded colonic tissue and multiparameter flow cytometry. Cancer Research. ,vol. 46, pp. 2428- 2434 ,(1986)
E. L. Kaplan, Paul Meier, Nonparametric Estimation from Incomplete Observations Springer Series in Statistics. ,vol. 53, pp. 319- 337 ,(1992) , 10.1007/978-1-4612-4380-9_25
WILLIAM A. KOKAL, ROBERT L. GARDINE, KHALIL SHEIBANI, PETER L. MORRIS, ELLIOT PRAGER, IRENE W. ZAK, JOSE J. TERZ, Tumor DNA content in resectable, primary colorectal carcinoma. Annals of Surgery. ,vol. 209, pp. 188- 193 ,(1989) , 10.1097/00000658-198902000-00009
O-P Kallioniemi, G Blanco, M Alavaikko, T Hietanen, J Mattila, K Lauslahti, T Koivula, Tumour DNA ploidy as an independent prognostic factor in breast cancer. British Journal of Cancer. ,vol. 56, pp. 637- 642 ,(1987) , 10.1038/BJC.1987.258
Robert S. Grinnell, THE GRADING AND PROGNOSIS OF CARCINOMA OF THE COLON AND RECTUM. Annals of Surgery. ,vol. 109, pp. 500- 533 ,(1939) , 10.1097/00000658-193904000-00002
D. J. Jones, P. F. Schofield, M. Moore, Prognostic significance of DNA ploidy in colorectal cancer: A prospective flow cytometric study British Journal of Surgery. ,vol. 75, pp. 28- 33 ,(1988) , 10.1002/BJS.1800750111
D W Hedley, M L Friedlander, I W Taylor, C A Rugg, E A Musgrove, Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry Journal of Histochemistry and Cytochemistry. ,vol. 31, pp. 1333- 1335 ,(1983) , 10.1177/31.11.6619538
William Kokal, Tumor DNA Content in the Prognosis of Colorectal Carcinoma JAMA: The Journal of the American Medical Association. ,vol. 255, pp. 3123- 3127 ,(1986) , 10.1001/JAMA.1986.03370220085032
N. C. Armitage, R. A. Robins, D. F. Evans, D. R. Turner, R. W. Baldwin, J. D. Hardcastle, The influence of tumour cell DNA abnormalities on survival in colorectal cancer British Journal of Surgery. ,vol. 72, pp. 828- 830 ,(1985) , 10.1002/BJS.1800721018
Wolfgang Hiddemann, Dirk B. von Bassewitz, Hans-Jürgen Kleinemeier, Elisabeth Schulte-Brochterbeck, Jan Hauss, Berud Lingemann, Thomas Büchner, Ekkehard Grundmann, DNA stemline heterogeneity in colorectal cancer. Cancer. ,vol. 58, pp. 258- 263 ,(1986) , 10.1002/1097-0142(19860715)58:2<258::AID-CNCR2820580210>3.0.CO;2-H